A simple, rapid, economic, sensitive and precise RP-HPLC method has been developed for the simultaneous estimation of dicyclomine and tramadol in capsule dosage form. An isocratic separation was carried out using C18 column (250 × 4.6 mm, 5 μm) and acetonitrile : 0.02M KH2PO4 Buffer pH 6.0 (±0.05) adjusted with 0.1M NaOH (70:30 % v/v) as mobile phase with quantification carried out at a wavelength of 215 nm. The retention time of the dicyclomine and tramadol was 7.77 and 2.89 minutes, respectively with theoretical plate count and asymmetry as per the ICH limits. The % assay of dicyclomine and tramadol were 99.99% and 101.07%. The flow rate was found to be 1 ml/min. The linear regression analysis data for the calibration plots showed a good linear relationship for dicyclomine and tramadol over a concentration range of 5-30 μg/ml and 25-150 μg/ml with correlation co-efficient of 0.9994 for dicyclomine and 0.9987 for tramadol. The limits of detection and quantification were found to be 0.07, 0.50 and 0.23, 1.51 μg/ml respectively.
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